Coding

Part:BBa_K1595022

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-13)

MBP with EcnB from Bordetella avium

T7 Promoter + Ribosome binding site + MBP + TEV + EcnB + 6x Hist tag + terminator. Coding device for anti-microbial peptide derived from Bordetella avium EcnB with a stabilizing maltose-binding protein and TEV cutsite.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1263
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1263
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1263
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138


[edit]
Categories
Parameters
None